Factors Affecting Fidelity of DNA Synthesis During PCR Amplification of d(C-A)n*d(G-T)n Microsatellite Repeats
نویسندگان
چکیده
منابع مشابه
A Novel Low Temperature PCR Assured High-Fidelity DNA Amplification
As previously reported, a novel low temperature (LoTemp) polymerase chain reaction (PCR) catalyzed by a moderately heat-resistant (MHR) DNA polymerase with a chemical-assisted denaturation temperature set at 85 °C instead of the conventional 94-96 °C can achieve high-fidelity DNA amplification of a target DNA, even after up to 120 PCR thermal cycles. Furthermore, such accurate amplification is ...
متن کاملFidelity of DNA polymerases in DNA amplification.
Denaturing gradient gel electrophoresis (DGGE) was used to separate and isolate the products of DNA amplification by polymerase chain reaction (PCR). The strategy permitted direct enumeration and identification of point mutations created by T4, modified T7, Klenow fragment of polymerase I, and Thermus aquaticus (Taq) DNA polymerases. Incorrectly synthesized sequences were separated from the wil...
متن کاملEffect of Lowering PCR Reaction Volumes and Increasing the Speed of DNA Amplification on the Sensitivity of PCR
ABSTRACT Recently, PCR is being reported more frequently with satisfactory results on the diagnosis of clinical infections. Widespread availability of PCR promises a sensitive and specific alternative, to traditional methods, but these benefits must be balanced against cost. Protocols using small volumes have described where the reaction taken place in capillary tubes, and small volumes work...
متن کاملSpermidine facilitates PCR amplification of target DNA.
Department of Agronomy and Range Science, University of California, Davis, California 95616-8515 Recent advances in PCR have made this technique one of the most powerful tools for a wide spectrum of molecular analyses, such as genome mapping, molecular evolution, diagnosis of genetic disease, and forensic sciences. ~ Many PCR applications involve the specific and reproducible amplif icat ion of...
متن کاملPCR amplification of long DNA fragments.
The polymerase chain reaction (PCR) has recently evolved as a standard laboratory technique, popular in all areas of molecular biology research. However, the technique still has two limitations: the relatively low fidelity of Taq polymerase when compared with other polymerases (1), and its inability to efficiently amplify fragments higher than 3 kbp (2, 3). Although these two issues are irrelev...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/24.12.2429